Print ISSN: 2309-5199

Online ISSN: 2616-6933

Issue 3,

Issue 3


Using of Broccoli Leaves on Diets of Common Carp (Cyprinus Carpio L.) and the effect as natural antioxidant and food additives.

Jafar Musa Saleh

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 1-15

This study was aimed at using powder of broccoli leaves as added food material and offsetting of industrial antioxidants used in synthesis of fish diets used in nutrition of carp fish (Cyprinus carpio L(.  . 60 little common carps were used in this study. Each plastic aquarium of the 12 aquariums. In this experiment, a semi-closed system was used and the aquariums were divided, according to the diet given,into 4 treatments each contains 3 replicates for each synthetic diet, including the control treatment  A, treatment B containing 5% broccoli powder (g /kg) and the treatment Cthat contained 10% (g / kg) broccoli powder as well as the treatment D that contained 15% (g / kg) broccoli leaves powder. All the manufactured diets were stored for 30 days under laboratory conditions to determine the effect of adding broccoli powder as an antioxidant in diets synthesized in the laboratory. The fish were fed twice a day to the point of satiety, and the environmental standards taken from aquarium water were within the suitable levels for the growth of common carp fish. and the values of environmental factors during the experiment period 25 ° C, pH 8.5 and the saline concentration 0.85  as well as dissolved oxygen 9.3 mg / L the effectiveness of broccoli powder used as an antioxidant was estimated by measuring the value of thaobarburture acid (TBA) for the fourth diets A, B, C, D, respectively, The results of the TBA test for the fourth diets showed significant differences (p <0.05) and the treatment D containing 15% broccoli powder.
Significant differences were also found in growth and food conversion rate as well as survival rate (p <0.05). The best growth of fish fed on diet C that was added with 10% broccoli leaves, and food conversion rate, The highest protein-to-energy ratio P: E was in treatment B and the lowest P: E ratio was in treatment D. the treatment C gave the highest survival rate reaching. The lowest survival rate was in the control treatment A .

Association of Growth hormone gene polymorphisms (T3199C) SNP on productive and physiological performance in Hubbard broiler chicken

I.Q AL-kreem; M. F. Muhammad; N.N. AL-Anbari

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 25-36

In this study commercial broiler hybrid (Hubbard) was used to detect the T3199C SNP in the fourth intron of chicken GH gene and investigate its effect on the productive and physiological performance in broiler chicken. PCR- RFLP method was used to identify this SNP. The results revealed that two genotypes were found when using of Msp1 restriction enzyme wild genotype TT and heterozygous TC. Highly significant difference (p<0.01) was found between the distribution of the different genotypes, the genotype TT had the highest percentage followed by TC and allele T had the superiority over allele C, no significant effect of the two genotypes on the productive traits in broiler breed of study, significant effect (p<0.05) of the two genotypes were found on the total protein concentrations of blood serum at 14 days of age and on the total protein and glucose concentration of blood serum at 42 days of age.

Effect of Growth hormone gene polymorphisms (T3094C) SNP on productive and physiological performance in Hubbard broiler chicken

I.Q AL-kreem; Ali abidalraheem

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 37-50

In this study commercial broiler hybrid (Hubbard) was used to detect the T3094C SNP in the fourth intron of chicken GH gene and investigate its effect on the productive and physiological performance in broiler chicken. PCR- RFLP method was used to identify this SNP. The results revealed that three genotypes were found when using of Msp1 restriction enzyme wild genotype TT, heterozygous TC and mutant CC. Highly significant difference (p<0.01) was found between the distribution of the different genotypes, the genotype TT had the highest percentage followed by TC then CC, allele T had the superiority over allele C, also significant differences (p<0.05) were found in mean of the weekly live body weight and weekly weight gain at 4, 5 and 6 weeks of age, and there was significant effect (p<0.05) of the T3094C SNP on the mean of carcass weight and relative weight of thighs between the different genotypes, the T3094C SNP was affect significantly (p<0.05) on the mean of blood serum total protein concentration at 14 days of age and on the mean of blood serum total protein and glucose concentration at 42 days of age .

Using goats and sheep follicular fluid with LHRH-a and some dopamine antagonist in spawning of common carp(Cyprinus carpio)

BAHA'A S. AMEER; KHALIL I. SALIH; HASHIM M. AL-RUBAEI

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 68-78

 The goats and sheep follicular fluid (FF), LHRH-a (Luteinizing hormone releasing hormone-analog), and some dopamine antagonists were used to stimulate common carp( cyprinus carpio) . The study included 24 females and 18 males and divided into 4 experiments. The results showed no significant differences between the females control group and the females treated in the first experiment, which used the FF for goats , motilium and a quarter of the amount of pituitary gland in its ratio and response time, total egg intake , quantity of eggs per kilogram of female and egg diameter with a reduction in injection cost 66.83%, while the males treatment were highly significantly ( P≤0.01) than males control of ratio and response time, total sperm volume, sperm volume per kilogram, and reduction in injection cost 87.64% .The results of the second experiment were significantly higher (P ≤0.05) than those of the control group on the treated females when the follicular fluid was used for goat, industrial hormone and metoclopramide, although the injection cost was reduced by 57%, while there were no significant differences between treatment and control males when used FF of goats and Metoclopramide with reduced injection cost of 88.21% . Also high significantly   (P ≤0.01) female control group in the third experiment injected with pituitary extract on females  treatment injected with follicular fluid for goat only .For the sheep follicle in the fourth experiment, the control females injected with the pituitary extract were significantly higher (P ≤0.01) than the females treated with follicular fluid for sheep, motilium and pituitary extract, while no significant difference was observed between males treated and control with reduced injection cost of 88.67% .

Effect of oxidative factor on quality of Frizian-holeshtine bull frozen- thawed semen and after thawed in different temperature and duration

.E.M.K AL-Najar; .H.F.M AL-Shemmary

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 79-92

The aim of the study is to evaluate the effect of oxidative factors on sperm quality (motility, viability, and abnormality) through the estimation of enzymatic antioxidant Superoxide dismutase (SOD) , Glutathione peroxidase (GPx) , and Total antioxidant capacity (TAC). And oxidative marker Malondialdehyde (MDA), and 8-Hydroxydeoxyguanosine (8-OHdG) in different thawing temperature and duration (37ºc for 30secnd as control group , 40ºc for 20second and 50ºc for 10second) to determine the optimum temperature and duration which improve the seminal quality and developed the ability of fertilization during Artificial Insemination(AI). The semen was collected from the Frizian-holeshtine bull in Abo-Graib AI center then; they have been diluted, packed, cooled and frozen according to the protocol of AI-center. 30 straws of each bull was supplied and divided in to 3 group to conduct physiological and biochemical test. The physiological had been conducted to determine the effect of thawing state of semen on the (Motility%, Viability%, and Abnormalities %). Biochemical test had been conducted on seminal plasma to measure (SOD, GPx, TAC, MDA, 8-OHdG).The result show significant increase (p<0.01) in the viability and motility (p<0.05) and significant decrease in abnormality , MDA and 8-OHdG concentration in thawing state of (50°c for10second) compared with control state (37ºc for 30second) which demonstrated high significant increase in motility, abnormalities  and (MDA, 8-OHdG , SOD , GPx and TAC) concentration. Also the study show negative correlation between (MDA, 8-OHdG) and motility, viability and negative correlation with abnormalities . Also show that there was positive correlation between (SOD, GPx, TAC) with viability and motility and negative correlation with abnormalities.

Effect of magnetized a nutritive Setter-2 on growth of some wheat varietes (Triticum aestivum L.)

Ahmed Fahem Jabbar AL-Jebbori

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 93-105

The experiment was carryout in the Fields of Agriculture College - Al-qadisiyah University during the winter season 2014-2015 using pot  capacity per 20kg as experimental unit which used the design of Random Complete Block Design R.C.B.D with three repeater, in order to know the effect of magnetized a nutritive Setter-2 on growth of some wheat varietes. Results showed the existence of significant influence of treatments in the Experiment excelled treatment latifia variet compared with the rest of the variet in the status of a length of plant, number of tillers, the number of leafs, leaf area, , number of spike and dry weight as it reached 55.4 cm , 4.47 , 15.58 , 51.0 cm2 , 4.87 and 40.28gm respectively while tammoz variet less than all at the characteristic in the experiment. It has a significant influence of magnetized a nutritive in most of the characteristic such as length of plant , number of tillers, leaf area, number of spike and dry weight it reached 53.9 cm , 6.67 , 43.7 cm2 , 4.60 and 37.76gm. but the interaction of treatments did not significant influence in most of the characteristic, nevertheless noted that the interaction between treatment latifia variet with the magnetized nutritive gave the highest results in most of the characteristic. Concluded from experience that there is a significant positive effect in most characteristic. So can use magnetic technical with the nutritive (Setter -2) of some of the variets to obtaining the highest characteristic of growth.

Study the effect of spraying of proline in the vegetative growth of the sun flower plant (Helianthus annuus L.) under different levels of water stress

B. A. Hameed Al- Da'mi

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 106-111

The study was carried out  using plastic pots in the Department of Biology- College of Education for Pure Sciences / Karbala University during the growing season (2015-2016) to study the effect of the interaction between different levels of water stress and proline acid in some growth indicators of the sun flower plant, which included (plant height , number of leaves, Leaf area, dry matter content and dry weight of root system).
Factional experiment within a completely randomized design (C.R.D.) with three replications was applied . The experiment included three concentrations of proline acid (0, 30 and 60) mg. L -1 and three levels of water stress (100%, 75%, 50%) of the field capacity. The experiment included 27 units (experimental unit). The results were statistically analyzed and the averages were measured using the least significant difference below the probability level of 0.05.
The results showed that the added water level of 100% of the field capacity value gave the highest values ​​for the studied traits (plant height, number of leaves, leaf area, dry matter content and dry weight of root system). It was also sprayed with proline acid with a concentration of 60 mg. L -1 gave higher values for the above-mentioned traits. Meanwhile showed bilateral interactions between the levels of water stress and proline acid showed a various effect on the studied traits.

Screening for the gene (GM1, GM4, GM11) disease gall midge re-sistance genes in Oryza sativa L cultivar using molecular indicators SSR

Z. Th. Abdul- Hussain AL-Refeay; N. Ab.H. Messan Al–Badeiry

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 116-129

Orseolia oryzae one dangerous pests that cause a great loss to the Oryza sativa L. crop, used markers Simple Sequence Repeats based polymerization reactions chained to the detection of resistance genes to disease. the DNA isolate of rice leaves  after 25 days from sowing .using Genomic DNA plant kit Protocol to extraction , the amount of DNA in good range (125-589) ng / μl  purity of 08.01 to 09.01. migrated samples on the gel akaros after completing the PCR program results showed three primers with sixteen cultivar ,The range of molecular size between (1156.84-197.768bp).the primer RM23956 reached to highest number of 16 alleles While the lowest number of 11 alleles recorded RM28574 primers. The Polymorphism information content (PIC) which is a reflection of the diversity of allelic and repetition allelic between varieties ranged value between ((0.8807-0.9837. As ranged gene  diversity values ​​between  0.8906- 0.9772. the primers RM23956 which reveals gane GM1 in twelve genotypes followed by the  RM28574 which reveals gane GM11 in seven genotypes either the primers RM547 which reveals gane GM4 in five genotypes, as the results showed that the  abaa1 cultivars of the contains three genes.

Analysis of the genetic diversity of Some rice varieties using microsat-ellite-markers technology

Z. Th. Abdul- Hussain AL-Refeay; N. Ab.H. Messan Al–Badeiry

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 130-150

genetic diversity of the eleven varieties of Oryza sativa L using molecular indicators collaboration with Mashkhab research center in the Najaf. Use polymerase chain reaction based the indicators Simple Sequence Repeats ,the DNA isolated from fresh leaves rice after 25 days from sowing using Genomic DNA plant kit Protocol . the amount of DNA in good range (125-589) ng/μl  purity of 1.8-1.9 . after agarose gel electrophoresis & staining with ethidium bromide. results showed that 64 alleles using 13 primers. The range of molecular size between (1015.402-115.423bp). RM3412 primer  reached the highest number of 8 alleles While the lowest number of alleles of 2 alleles recorded RM201,RM10772 primers. The Polymorphism information content (PIC), which is a reflection of the diversity of allelic and repetition allelic between varieties ranged value between (0.0830 -0.8434) . As ranged genetic dimension values ​​between) 0.0868- 0.8595 (. Record all of the primer RM3412 higher values ​​ Heterozygote reached 1. this primers (RM3412 and RM8085) to give a distinctive genetic fingerprinting of three varieties under study. The lowest genetic distance 0.3269 between varieties 2 and 3 this means there is a high degree of similarity. The genetic similarity of all varieties and compositions ranged betwee ) 0.1154 - 0.6731) depend on the genetic distance that ranging from) (0.8846 -0.3269) which refers to the large genetic diversity ranged from(88% -32%) (and this reveals a high genetic variation between varieties  making it hereditary rich sources. Results showed of the Cluster analysis to 2 major groups (Main Cluster) at the level of the similarity of  0.12.

Qualitative and quantitive identification of some secondary compounds in the bark of (Salix alba l.) Naturally growing in dohuk by using HPLC .

Talal K. Al-Takay; Ayad C. Al-Daody; Walid A. Kasir

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 151-159

Separation of some secondary compounds in the bark of Salix alba L. trees was carried out. Sequence extraction with two different polar solvents (Petroleum ether as a non polar solvent and Ethanol 95 % polar solvent) was done using Soxhlet apparatus in order to prepare the crude extract. High performance liquid chromatography (HPLC) was used for chromatographic separationidentification and also determination of some secondary compounds and its percentages. The results were showed a remarkable variation in the kind, number, percentage and the peak area (represented the concentration) of each separated compound. Citric acid, Acetylsalicylic acid and also Cinnamic acid were identified. Acetylsalicylic acid (Aspirin) was isolated for the first time from the bark of Salix alba L. trees .

Evaluation of the antioxidant activity to the nanoprepared propyl gal-late.

Mais Ibraheem; Zahraa Murshidy; Ali Al-Ghanimi

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 160-172

A nanohybrid compound PG-ZnO was prepared using sol-gel ion exchange method between the antioxidant propyl gallate and zinc oxide . The new nanoantioxidant compound was identified. FT-IR spectroscopy revealed the appearance of new groups in the PG-ZnO spectrum which indicates the formation of new antioxidant . The X-Ray Diffraction (XRD) spectrum revealed the appearance of new diffraction planes in the PG-ZnO spectrum in compare with the spectrum of zinc oxide . The antioxidant activity of PG-ZnO was studied using free radical scavenging method. Result revealed that IC50 were (75 and 25) µg/ml for the nano and free antioxidant , respectively.

Bioactivity of some natural extracts on the life of black bean aphid. Aphis fabae ( Scop). Aphidadae : Homptera.

Mohsen AL-Musaifer

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 173-183

A field and laboratory study were conducted to determine the efficiency of ethanolic plant of Matricaria chamomilla  and propolis in life performance of the aphid black bean, where the study showed superiority propolis in the prevention of hatch and the peril of the nymphs and adult cumulative rate of (37.46, 70.24, 62.45)%, respectively. While it decreased in the effect of Matricaia chamomilla in the prevention of hatch and the peril of the nymphs and adult cumulative rate of (60.22, 45.51, 52.72)%, respectively. The study also showed a significant superiority of the concentration of 3% of the propolis to prevent hatch and average cumulative peril of nymphs and adult was (10.0, 100.0, 97.70)%, respectively. While the concentration of 3% of Matricaia chamomilla less impact in the prevention of hatch and average cumulative at peril of nymphs and adult was (31.06, 77.73, 77.73)%, respectively .Compare with the control treatment, in which the birth rate and the peril of the nymphs and adult was (96.63, 3.3, 3.3)% to respectively.
The study also showed greater impact by increasing the time period was the third day after treatment of Matricaia chamomilla and a clear role in the prevention of hatch and the peril of the nymphs and adult cumulative rate of (54.12, 49.12, 57.45)%, respectively. While the same time period to propolis gave better effect in the prevention of hatch and decimation nymphs and adult cumulative rate of (30.80, 74.12, 67.45)%, respectively .Compare with the treatment given to control where give rate at a cumulative was  (100.0, 3.3, 3.3)%, respectively.

Effect of Bacterial Inoculation and Phosphate Fertilization in growth Lathyrus sativus L.) plant ) of and production

Fadhel S. Al – kinany

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 184-193

Afield experiment was conducted in agriculture field of extension center Mhanawia – Nourth babyl. Alocal cultivar of grasspea was planting during autumn season of 2015, and RCBD with three replication was used. Two level of bacterial inoculation ( with and without inoculation ). Moreover phosphate fertilizer ( 0 , 50 , 100 KgP2O5 . ha-1 ) in the form of triple superphosphate Ca(H2PO4)2 (P2O5 45%) was used. Means compared using the Least significant Differences ( L.S.D ) test at probability level of 5%.
   The Results showed that addition of phosphor Fertilizer at level 100 Kg P2O5.ha-1 excelled significantly in the most of Qualities studied ( plant heigh , number pods, number of seeds pod, production , number and dry wheight of active nodes and available Nitrogen in soil after plantig ) as it gave average respectively    ( 55.1cm ,    24.6 pod.plant-1, 9.6 seed. Pod-1 , 1117.1 kg.ha-1 , 15.7node. plant-1 , 63.1 mlg. plant-1 , 42 mlg.kg-1 ).
    The Results showed that bacterial inculation gave  excelled significantly in all Qualities studied ( plant heigh , number pods, number of seeds pod , wheight of 1000 seeds , production , number and dry wheight of active nodes and available Nitrogen in soil after plantig ) as it gave average respectively ( 54.4 cm , 24.8 pod.plant-1, 9.1 seed. Pod-1, 64.1g , 1764.9 kg.ha-1 , 20.3node. plant-1 , 90.1 mlg. Plant-1 , 45 mlg.kg-1).
Results showed that the overlap between study facters ( phosphate fertilization and bacterial inculation ) at level 100 KgP2O5.ha-1 with bacterial inculation  also gave excelled significantly in the most of Qualities studied ( plant heigh , number pods, number of seeds pod , production , number and dry wheight of active nodes and available Nitrogen in soil after plantig ) as it gave average respectively     ( 62.9cm ,    28.4 pod.plant-1, 10.8 seed. Pod-1 , 1935.8 kg.ha-1 , 23.9 node. plant-1 , 100.40 mlg. Plant-1 , 51.3 mlg.kg-1 ).

Isolation and Identificution of Cryptococcus neoformans from some Environmental resources in Najaf covernorate

Fatima A. Majbeel; Nabeel S. Majeed

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 1-11

The aims of the present study were to isolate and identificate of isolation and identificution of Cryptococcus neoformans from some environmental resources in Najaf covernor  associated with fungal infection .C.neoformansrepresents one of the isolated fungi from environmental resources samples, which may cause many serious problems to human and in immunocompromised patients inparticular.
Laboratory isolation and identification of C. neoformans a total of (150) environmental resources samples are collected from (6) different areas; (100) samples are obtained from pigeon droppings and (50) samples from soil surrounding the Eucalyptus sp. trees and decaying wood were collected, during 2015.
The positive results of cultures (112) (74.66%) isolates of C. neoformans were isolated from samples of pigeon droppings and isolates from soil sourrunding the Eucalyptus trees and decaying wood samples.
The cultural and biochemical methods were used in Cryptococcus spp. Isolation and identification, besides VITEK2 compact system identification kit and CHROM agar candida medium. Also identification by VITEK 2 compact system, showed two speices C. neoformans (12) samples and C. gattii (3) samples.
The best trust level appeared within potentiality rate of 93%, however the other isolates had a fewer potentiality rate reached 85% and this level was accepted . Cryptococcus was identified by conventional tests and yeast which show positive results for the capsule test, urease test, melanin pigment when cultured on methyl dopa culture for a period from 18 – 24 hrs. Then confirmed by VITEK2 compact system identification kit. The results of CHROM agar candida medium test are confirmed the results of the VITEK 2 compact yeast identification system. In this test, the colony color of Cryptococcus spp. on the medium are pink for C. neoformans

Effect of Addition of Humic Acid on broiler chickens diets on some the performance and carcass traits

Salah M. Kati

Journal of Kerbala for Agricultural Sciences, 2017, Volume 4, Issue 3, Pages 51-67

The experiment was carried out in the poultry  at Babylon to study the effect of Humic Acid in the diet on performance of Ross 308  Broiler chicks   During the first period which extended form the 1st to the 6th week The period From 20/1/2017  to 5/3/2017 . A total of 180 one day old chicks were used , chicks were allocated randomly in to 4 equal treatments, 45 chicks for each treatment , each treatment was included 3 equal replicates, 15 chicks for each one. Treatments were arranged as follow:
      The T1 treatments fed of  Basal diet (Control group) and  T2 treatments fed supplemented with Basal diet + 2.4 g/kg Humic Acids  and T3  treatments fed supplemented with Basal diet + 4.8 g/kg Humic Acids  and  T4  treatments fed supplemented with Basal diet + 7.2 g/kg Humic Acids  .
   Results showed the following ; Addition of Humic Acids in the diet Broiler chicks during the period (1-6) week of the experiment , had a significant effect ) P≤0.05 (on body weights of Broiler chicks and accumulative feed consumption , accumulative weights gain and feed conversion rate as compared with the control treatment. Addition of Humic Acid significant effect  on Carcass traits (Dressing percentage , The relative weight of the various Parts and Internal organs )  in comparison with control treatment .