Print ISSN: 2309-5199

Online ISSN: 2616-6933

Issue 3,

Issue 3

Effect of Foliar Application of Nutrient Solation [Marval] and Salicylic acid and Their Interaction on Flowering and Chemical Characteristics of Jujube Tree Zizphus mauritiana Lamk. cv. Tayahi

Nada A. Ameir; Khawla H. Muhammad; Aqee l H. Abdulwahed

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 1-12

The present experiment was carried out in a private orcharal, Al-Hartha north of Basrah during the growing season of 2015. Ten year old jujube trees cv. Tufahi were used. The tree were sprayed with the nutrient solution Marvel at (0, 4 and 8)ml.l-1 and Salicylic acid at (0, 50, 100 and 150)mg.l-1 and their interaction. The trees were sprayed twice with an interval of one month the effect of these treatments on flowering and chemical characteristic studied.
The results showed that treatment with the nutrient solution (marvel) caused a significant increase in all flowering characteristic (length of flowering stalk, number flower cluster) and chemical characteristic including N, P, K and Fe leaf content. The results also showed that’s spraying with SA giving 150 mg.l-1 caused a significant increase in flower and chemical characters as for the interaction effect on trees sprayed with 8 ml.l-1 Marvel + 150 mg.L SA recorded the highest studied characteristics

Effect of Organic and Bio-fertilizer on some yield and quantity parameters for two strawberry varieties Fragaria× ananassa Duch

Fouad Abass Salman; Ayad Jasim Jaber

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 13-30

 The study was conducted in Horticulture and Forest Unit at Al-Najaf Agriculture directorate during 2016-2017 growing season, to study the effect of adding some Bio and Organic fertilizer on yield and some quality parameters of strawberry (Fragaria ananassa Duch) cultivated in plastic house.
     The first factor included two varieties of strawberry: Albion and Burtela while the second factor was adding seven treatments of organic and bio-fertilizer including: without adding fertilizer (control), adding Azotobecter chroococcum (10g.plant-1), adding of Bacillus subitls (10 ml. plant-1), spraying of organic fertilizer Appetizer (1 ml. plant -1), adding of A.chroococcum + B. subitls, adding A.chroococcum + spraying of Appetizer, adding B. subitls + spraying of Appetizer and adding of A.chroococcum + B. subitls with spraying of Appetizer. Factorial experiment using Randomized Complete Block Design ( R. C. B. D) with three replicates were used.The Duncans multiples range test at 0.05 probabilities was adopted to compare the treatments means.  
     The Bortila variety was significantly increased the fruits number to 27.08 fruit.   Plant -1, weight fruit rated to 25.05gm.fruit-1 and yield to 675.50 gm. Plant-1.The type of fertilizer showed a significant increase with adding A.chroococcum + B. subitls + Appetizer sprayed fruit number to 30.00 fruit.plant-1, and fruit weight rated 27.97gm.fruit-1 The Albion variety markedly increased total sugar to 10.54 mg.100gm fresh weight-1, while the Burtela variety showed significantly increase total acidity to 4.29mg.100g fresh weight-1and ascorbic acid to 33.73 mg.100gm fresh weight-1.
The fertilizer of A.chroococcum + B. subitls + Appetizer sprayed significantly increased total sugar to 12.11 mg.100gm fresh weight-1, while nitrate content reduces to 14.13mg.100gm dry weight-1. The treatment of A.chroococcum + Appetizer sprayed significantly increased Ascorbic acid to 55.47 mg.100gm fresh weight-1. The control treatment showed significant increase in total acidity 5.35 gm.100gm fresh weight-1 and nitrate content to 19.45 mg.100gm dry weights-1.

Analysis some of chemical pesticides by species of Aspergillus spp. fungi in soils and various organic fertilizers

Sadeq Mohammed Ali; Salwan A. Zahra Jabbar Allobawi; Adeeb Kitab Abid Zaid Alshafiee; Huseein Rasem Sabory

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 31-44

This study aimed to analyzing some pesticide residues using Aspergillus species ,five types of chemicah pesticides are used  ;fungicides  (Ridomil Gold, Esther Aster and Nomolt ,(Insecticides) Chevalier and Palace Paalas ) Herbicides  which are used in the controlling some pests of wheat where the effect  of pesticides on the solid and liquid agricultural mediums  was measured (PDA and PDB) the results showed that the herbicide Chevalier has significant differences on the fungus( A.parasiticus,A.flavus.) ,where the percentage of inhibition (100.00)%, respectively while solid medium ,The rates of inhibition of growth of fungal mass were in the broth medium( 17.69 ,97.97) respectively  ,However the herbicide Paalsa has significant impact on the fungi(A.terres.) ,Where the percentage of inhibition (98.60) %  in the solid medium , the  percentage of inhibition of the fungal mass growth was reached( 7.50)% .

Effect of Salicylic acid on the antioxidant enzymes activity for callus of grape Vitis vinifera L. Halawini under salt stress


Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 45-58

The experiment aimed to study the effect of adding Salicylic acid in salinity stress on callus of grape Vitis vinifera L. Halawini.
It was achieved in the laboratories of plant tissue culture belong to Horticulture and leaned scabe faculty of agriculture, University of kufa. during a period from October 2017 to December 2017. 250 mg of grape callus Halawini cultured under the effect of  salicity stress in culture medium MS with sodium chloride  (NaCl) (0, 75 and 150 mM) respectively with the adding  salicylic acid(SA) on contractions (0, 0.5 and 1 mM). The culturing period was 8 weeks. The results revealed a significant effect for salicylic acid adding in lowering oxidative stress enzymes namely superoxide dismutase (SOD), catalase (CAT), Ascorbate  peroxidas (APX) and peroxidase (POD).

Study the efficiency of pheromone traps with virgin female on attracting male of Chrysomya megacephala( Diptera: Calliphoridae)

Nassir A. Al-jamali; Ali A.H. Al-Sudani

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 59-67

Chrysomya megacephala secondary myiasis fly is one of insects that has medical and veterinary importance in the world which caused secondary myiasis to stinking  decomposing animal wounds , in order to study the efficiency of pheromone traps with virgin females to attract it,s male,it is important to determine the factors affecting the efficacy of pheromone traps when they are used to monitor and control  C. megacephala fly. This study was conducted at Frehaa region, 4 km North-east of Karbala city . The results showed the best number of virgin females were (5)which gave 0.76 insect/trap/day Compared with 0.42 and 0.33 insect/trap/day at traps which  provided 1.0 and 10.0 virgin female Respectively. The best hight  to hang trap was 1.5 m with 1.27 insect/trap/day Compared with 0.55 and 0.61 insect/trap/ day the traps which at hight 0.5 and 1.0 m respectively  while the best distance between pheromone traps was 50 m to prevent the interaction between traps which gave 0.77 insect/trap/day compared with 0.38 , 0.27 insect/trap/day in distances 100 , 150 respectively  .the result showed that the activating attracting period were 4 days, must be replaced with anther virgin females with     2 days old  to complete it,s sexual maturity in order to be able to attract it,s males.

Biological and Chemical control of Fusarium solani caused by disease Root Rot Zea mays

Olaa H. jaefar

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 68-76

This study was conducted to investigate the effect of Tatroviride., Chaetomium spp, bread yeast Saccharomyces cerevisiae, and salicylic acid on F.solani, isolated from the roots of yellow maize plants. Atrophiride T.atroviride and Chaetomium SpP achieved high efficiency in inhibiting F.solanigrowth on the PDA medium. Yeast was also added to the PDA medium at the concentration 1 g. L-1 inhibition of 68.55%. The factors used in the study all achieved a high degree of reduction in the percentage of the total root injury. Salicylic acid was added with a concentration of 100 mg. kg-1 soil in the first place with a root percentage of 8.33% Yeast then the coefficient Acid Alsalcelik concentration of 50 soil and then treated biological fungi Chaetomium spp and T.atroviride The yeast treatment was characterized by the presence of fungus L. F.solani and the lengths were (9.25 and 8 Cm) followed by Salsalic acid treatments and then the two biological fungi Chaetomium spp and Tatroviride compared to the length of plants with the presence of fungus F.solani alone in which the lengths were (4 and 3 cm).

Isolation and diagnosis of some fungi associated with surface and digestive of Periplaneta americana L.(Orthoptera: Blattidae) in Karbala governance and Potential Biological Control of them

Hussein Ali Baqir

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 77-89

This study was conducted in Karbala governance‏ during 2014, for isolation and identification of some fungi from surface and digestive of Periplaneta americana L.(Orthoptera: Blattidae). The insect samples were collected from women's and obstetricians hospital and residential areas near of the hospital. The study showed isolation and identification several fungi that were Aspergillus niger, A.fumigatus , Trichoderma harzianum , Penicillium sp. , Alternaria sp. , Mucor sp. and Fusarium sp from surface and digestive of P. americana. The Aspergillus niger was the highest percentage frequency about 26.32%, compared with other fungi while the lowest percentage frequency was recorded for Mucor sp. with 1.75%. The pathogenicity test indicated to the potential effect of Trichoderma harzianum against adult of P. Americana. The T. harzianum suspensions were applied on the adult either by direct spraying or as bait. The bait method reached mortality 80% of the insect with fungal concentration 17x 107 after 10 day compared with direct spraying that was reached 40% mortality with same fungi in concentration above after same period.

Effect of hornwort extract and magnetically treated water on some growth chemical and production of cucumber cucumis sativus l. In desert area

Manssoor A. Aboohanah; Ali F. Al Amin

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 90-105

   Field experiment was conducted in An-Najaf governorate during of 2016 in two season spring and autumn. The aim of this experiment was to study the effect of hornwort extractions and magnetically treated water on growth and yield parameters in cucumber plant beta alfa variety
         The experiment included nine treatments, i.e. the interactions of  three concentrations  of hornwort extractions (first factor)(0, 10 and 20ml.L-1)sprayed on vegetative and magnetically treated water(second factor) (0, 1000 and 2000 gauss).Sprays were applied third, first sprayed through four true leaves stages, second flowering initiation, third after two weeks from second sprayed.
Split-Plot Design with in Randomized Complete Block Design (R. C. B. D.)  was used with three replications. magnetically treated water treatments was distributed in main plots and hornwort extractions treatments in sub plot , Means were compared according to Duncan's Multiple Range Test (D.M.R.T.) at probability of 0.05.
     Results can be summarized as follows:.
1- Using of concentrations  of hornwort extractions had a significant effect on measured vegetative growth parameters (plant length, leaves number). There was no significant effect between 10 and 20ml.L-1in plant length parameter. There was no significant effect between1000 and 2000 gauss in plant length for two season and leaves number in spring cultivation. interaction between factors gave the same trends on vegetative growth parameters.
2- Using of concentrations  of hornwort extractions had a significant effect on fruit growth parameters 20ml.L-1treatment gave the highest means for the fruit length and total yield (53.08 and 39.55ton.h-1)  compared with control gave the lowest means for the measured parameters for both season. There was no significant effect between 10 and 20ml.L-1 in fruit length parameter in spring cultivation,2000 gauss treatment gave the highest means in total yield(48.51 ton.h-1) in spring cultivation while1000 gauss treatment gave the highest means in total yield(35.70 ton.h-1) in autumn cultivation, 1000 and 2000 gauss don’t gave a significant effect in total yield for both season.1000 and 2000 gauss don’t gave a significant effect in fruit length for both season.
3-Spraying  of hornwort extraction as conc. 20ml.L-1 had a significant effect on measured chemical parameters such, total chlorophyll for both season, respectively, while proline in leaves which gave the least values with the use of hornwort extraction.
         Irrigation with 2000 gauss treatment  gave the highest means for the, total chlorophyll for both season, while  proline in leaves which gave the least values with the use of magnetically treated water. There was no significant effect between 1000 and 2000 gauss in total chlorophyll content for both season and proline in leaves in the Spring cultivation.

Effect of the Spray Amino Acids and Drip Irrigation Depth on Growth and Yield of Egg Plant (Solanum melongena L.)

Hayder Sadaq Jaafer

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 106-115

An experiment was conducted in a plastic house in Horticulture and Forestry Department belongs Al-Najaf Agricultural Directorate during the growing seasons of 2015-2016. The aim was to study the effect of the spray amino acids and irrigation depth on growth and yield of egg plant (Solanum melongena L.). The experiment had comprised twelve treatments; three concentrations (0 and 2 and 4) ml.L-1 of amino acids and four depth of water applications surface drip irrigation D0 and sub-surface at depth of 4 and 8 and 12 cm namely D4 and D8 and D12, respectively. Variations between the means were tested according to Duncan's Multiple Range (D.M.R.)   at probability of 0.05.  
     Results showed that spraying with amino acids or add irrigation water with different depths improved vegetative growth (plant height and leaf area and dry root weight and total vegetative dry weight), and total yield. Interaction amino acid spraying with 4 ml.L-1 with treatment of depth 4 cm gave the highest values compared with the interaction treatment of control with treatment of depth 12 cm which gave the lowest values.

Effect of Reef Amirich spraying in growth and yield two caltivers of cabbage

Khalid Abed Mater Al-lamy; Mohammed Hade Abeed; Mahdy Abd-alaziz Sakr

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 116-123

An experiment was carried out in one of the farms of Horticulture Department – College of Agriculture – Karbala University during 2014 – 2015 growing seasons aimed to study the effect of Reef Amirich spraying(S1) and without spraying(S2) in growth and yield of two caltivers of cabbage Copenhagen market(V1) and Ruby queen(V2). Randomized Complete Block Design (RCBD) with three replicates were used.
The results showed that spraying of Copenhagen market (V1S2) gave a significant increases in plant height(13.42 cm) and total leaf area(105.28 dcm2.plant-1) compared with most other treatments, while highest values in chlorophyll content of leaves recorded in V2S2 treatment(79.13 SPAD), whereas V1S2 treatment gave a significant increases in yield of heads(2.060 kg. plant-1), while  V2S1 treatment gave a lowest value(1.330 kg. plant-1). 

Effect levels energy and protein and type feed on Broiler performance Ross.

Abbas Salime Hussein Al-Machi

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 124-136

This experiment aimed to evaluate the replacement of some materials locally available used in the manufacture of feed mixtures  instead of  pre-prepared imported and used in the production of hens meat, this study was carried out at the Poultry Research Station –department of Karbala agriculture - Karbala Governorate at the period from 15/11/2016 to 27/12/2016 to study the effect change  different levels from Energy and Protein and type feed Pellet and Mash in broiler performance, in the experiment, 1200 hens meat was used (ross variety) one day age and weight 42gm.chick-1, Purchased from one of the commercial hatcheries in the province of Karbala, chicks were randomly distributed to four treatments (300 chicks for treatment) with three replicates (100 chicks for replicate) experiment started since first day of age to 6 weeks age, CRD design used to data analysis, the treatments was divided as follows: T1 (control) : broiler feeding by the feed Mash. T2 broiler feeding by the feed Pellet with energy and protein  agreeing with Gide Breeding. T3 :  T2 broiler feeding by the feed Pellet with level energy and protein like T1 (control) but it is apellet.T4 : T2 broiler feeding by the feed Pellet with low energy of T2 about 50 Kcal and protein  like same T2 . The productivity characters were studied included body weight, the increase in weight, feed consumption, feed conversion coefficient of hens, mortality rate, dress percentage, percentage of internal organs weight and percentages of the main and secondary parts of the carcass. The results showed the following: There were significant differences at (P ≤0.01) in rates of live body weight, weight increase, feed consumption increased, percentage of mortality rate, relative weight of the back, The lowest cost of feed was when feeding by T2 treatment.

Relationship of some metabolic and hormonal compounds in the blood with the productive and reproductive performance in local Awassi ewes

Hashim M. Al-Rubaei; Makki Khalaf Hussein; Ali Jasim Jafar

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 137-150

The study was conducted at AL-Fayhaa station in Jabila province (50 km North of Babil) and laboratories of the Department of Animal production techniques for the period from February to June to show the relationship between some metabolic and hormonal compounds in the blood with production and reproductive performance in local awassi ewes. Data were collected from 50 milking ewes 2-5 years old from 220 records of daily milk production for fertility percentage and prolificacy rate. The results showed that the regression coefficient of daily milk production on total protein (dl) and their coefficient reach to 0.0057 and prolactin hormone (ml) were positive and highly significant  (P<0.01) and their coefficient reach to 0.019 and on glucose concentration (dl) were negative and highly significant (P<0.01) and their coefficient reach to - 0.028 and on total cholesterol concentration (dl) were negative and significant (P<0.05) and their coefficient reach to - 0.0053 The regression coefficient of fertility percentage on follicle stimulation hormone was observed positive and highly significant (P<0.01) and on triglycerides concentration were positive and significant (P<0.05) . the result also showed that the regression coefficient of prolificacy rate on glucose and total protein concentrations were negative and highly significant (P<0.01) . the correlation coefficient between metabolic and hormone compounds in the blood with production and reproductive performance for ewes were supported to the regression coefficient of same traits .we conclude from this study the possibility of using metabolic and hormonal compounds in rapid selection programs to improve head of ewes     .  

Studying of genetic and phenotypic variances, correlation and path coefficient analysis of yield and its component in some wheat varieties

Faez Fayad Mohammed Alogaidi

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 151-163

A field experiment was conducted in Dhi-Qar Governorate during winter season 2015-2016 to study genetic and phenotypic variation and genetic, phenotypic and environmental correlations, broad sense heritability and path coefficient for nine traits: plant height (PH), flag leaf area (FLA), tiller number (TN), spike number (SN), spike length (SL), grain number in spike (GN/S), 1000 grain weight (GW), biological weight (BW), grain yield (GY) in three wheat varieties: Aab’a99, Barcelona and Boro. The results showed that the values of phenotypic variance coefficient were close to those of genotypic variance coefficient for most traits: PH, TN, SN, GW, FLA, BW and GY. Thus the genotype can be studied through phenotypic data, and such traits can be considered as selection criteria in wheat breeding. The highest ratios of broad sense heritability were for PH, TN, FLA, BW and GY (0.83, 0.8, o.87, 0.86, 0.82 and0.95) respectively. Phenotypic correlation coefficients of all studied traits were positive and significant and generally were lower than genetic correlation coefficients. This indicates that variations among varieties were largely of genetic origin despite the existence of low environmental effect. Genetic correlations of GY in SN and GN/S traits were positive and the highest (0.941, 0.902) respectively. The highest direct effects on grain yield were of SN and FLA (1.283, 0.993) respectively. Therefore, Path coefficient analysis reveals that the direct selection of these two traits will be helpful in breeding programs for improving grain yield in wheat crop.

Trend Of Graduates Of Agricultural Disciplines and their Impact on the received Status of Business Organizations (A survey study of the Opinions of a Samples of traders with the Directorate of Agriculture in Najaf Governorate )

Saleem M. Ameen; Arshad A. Ameer; Abdulnaser A. Alsayyed

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 164-196

The trend is Consider one of the terms that the researchers depend upon it in their studies specially in the field of psychology, because it has a clear effect on changing activity and of individual to that activity. As the activity of agricultural field is Considered from vital tributaries which the government has to pay attention to them, especially as many countries of the world are flourishing and growing in this filed,  they need to do a lot of work to  Support this vital sector, through supporting the academic institutions to train the future skills, to maintains this activity to go with development and environmental changes, within this sector. In addition to that, the government should work to find business organization that provide  facilities and support to this sector such as agriculture directorate specialized  agricultural bank to provide its  requirements.
  It is worth mention if those organizations want to remain a competitive work environment and grow in a dynamic and so histicuted  environment and a complex communication network,  the must meet the needs of their customers and obtain their satisfaction  either it is a  service or a commodity  provided to them to occupy these organization their perceived  status.
So, the importance of this study has emerged  through the effect of the components of trend of agricultural graduates disciplines  to show the perceived states of business organizations in particular at level of country in  general by identifying those components as well as identifying the dimensions of perceived status in line with trends of individuals.
The study concludes that the knowledge ranked first by trend Component influences on the highlight of  perceived states of studied organization by its  behavioral dimension which linked first among its dimensions from the point of view of the sample.

Effect of addition of different levels of humic acid to drinking water on productive and carcass characteristics of broiler chick

Salah M. Kati

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 1-13

  This experiment was carried out at the  which poultry farm  of  Babylon to study the effect of adding humic acid to the drinking water on performance of Ross 308  broiler chicks . The experimented the period from  20/1/2017  to 5/3/2017of birds age  , A total of 135 one day old chicks were used in this experimented , chicks were allocated randomly in                     to 3 treatments, 45 chicks for each treatment , each treatment was included 3 equal replicates, 15 chicks for each one. 
The treatments , were: -
Treatment (T1)  basal diet + fresh water without addition (Control group).
Treatment(T2)  basal diet + fresh water supplemented with Humic acids in  ( 3.6 g/l liter water .
Treatment(T3)  basal diet + fresh water supplemented with Humic acids     ( 7.8 g/l liter water .
       The results of this experiment indicated that  supplemented of Humic acids in the fresh  water  during the period (1-6) week of the experiment , had a significant effect ) P≤0.05) on body weights rate of chicks and accumulative feed consumption , accumulative weights gain of the birds  and significant effect) P≤0.05) of feed conversion rate as compared with the control treatment. Addition of humic acid has an significant effect of the birds  ) P≤0.05) on the carcass traits  of birds (The relative weight of the various carcass cuts  and Internal organs )  in comparison to the birds of  control treatment.

Hasan Abdullah Mohammed

Hasan Abdullah Mohammed

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 14-22

This work done in  farm kosh Taba closed Erbil city from 20/4/2016till 25/5/2016. Aimed this experiment to seeking effect of supplemented  Individual and combining powders of Nigella sativa and Thymus vulgaris leaves. A total number of 200 one day old straight run broiler (Ross-308 hybrid) chicks were divided to four dietary treatments each of treatment with 5 replicate, each of replicate has 10 chicks, the control group (C) without any supplement sources treatment one (T1) supplement 0.50 % of powder Nigella sativa seeds, treatment two (T2) supplement 0.50 % of Thymus vulgaris, treatment three (T3) supplement mixing of 0.25% powder Nigella sativa seeds and 0.25% of Thymus vulgaris both. This study focused on effect supplement two type of herbs on determination of chemical composition for main carcasses parts muscle (breast and thigh).  Results observed significant (P≤0.01) among all treatment. High value for moisture, protein, fat and total cholesterol was in T3, T2, C and C respectively, while insignificant (P≥0.01) among all treatments for Ash. For thigh with same parameters results observed significant (P≤0.01) with all treatment high value for moisture, protein, fat and total cholesterol was in [T1, T2, C and C, T3 respectively, while insignificant (P≥0.01) among all treatments for Ash.

Effect of some biological agents and chemical fungicides to induce systemic resistance in cucumber plants against Fusarium oxysporum f.sp. cucumerinum

Ali F. M. AL-Hujazy; Saleh H. Sameer

Journal of Kerbala for Agricultural Sciences, 2018, Volume 5, Issue 3, Pages 23-39

The aim of the study was to evaluate  the efficiency of some biological agents and chemical fungicides to reduce disease percentage and disease severity of Fusarium wilt disease in cucumber, which caused by Fusariumoxysporum f.sp. cucumerinum under plastic house conditions, and detection of the effectiveness of factors induction through the investigation for activity peroxidase enzyme , the amount of phenols as well as the amount of total chlorophyll in cucumber plants leaves . Results showed superiority treatments of biological agents Trichoderma viride , Trichoderma harzianum , bio-preparation Biohealth and preparation of bacterial  Fluramel (alone or in mixture). Treatments reduced disease percentage and disease severity to 0 and 0 % respectively, compared to control treatment (pathogen) was 86.67 and 68% respectively. Results showed  that after 7and 14 days of the addition pathogenic fungus to cucumber plants , was superior treatment of interaction between Biohealth with  chemical fungicide (Uniform) in the presence of pathogen in the increase activity of peroxidase enzyme where amounted to average absorption 7.17 and 5.89 min/gfw respectively . Also the same treatment superior in recording highest phenols in leaves cucumber plants after 7and 14 days of the addition treatment  pathogenic fungus to cucumber plants to 7.77 and 7.38 mg/gfw respectively, while where amounted to treatment of pathogen  2.63 and 2.48 mg/gfw respectively , and superior treatment of Biohealth on the other treatments in increase total chlorophyll after 7and 14 days of treatment with pathogenic fungus to cucumber plants to 38.3 and 36.5 Spad respectively, while in treatment of pathogenic fungus recorded 27.7 and 24.4 Spad respectively.