Journal of Kerbala for Agricultural Sciences, Agriculture of College, Kerbela University Journal of Kerbala for Agricultural Sciences 2309-5199 5 2 2018 06 01 This study was carried out in the Plant Virology Laboratory belonging to the Plant Protection Department at the Faculty of Agriculture-Karbala University with the aim of isolation and molecular identification of five fungi, isolated from the roots of some التشخیص الجزیئی لعزلات تابعة لمفطریات Rhizoctonia solani و Fusarium verticilliodes و Fusarium solani و Pencillium tardochrysogenum المعزولة من جذور بعض نباتات الطماطة L.) lycopersicum Solanum( 41 59 160239 EN Aqeel Nazzal Barbar College of Agriculture-Karbala University Ahmed B. Abu-Duka Department of Plant Protection, College of Agriculture, University of Karbala Noor A. AL-Ghazali A. AL-Ghazali Department of Plant Protection, College of Agriculture, University of Karbala Amel A. Ali Department of Plant Protection, College of Agriculture, University of Kufa Journal Article 2019 05 08 This study was carried out in the Plant Virology Laboratory belonging to the Plant Protection Department at the Faculty of Agriculture-Karbala University with the aim of isolation and molecular identification of five fungi, isolated from the roots of some tomato plants growing in the plastic tunnels located in a desert region in Karbala governorate during the agricultural season 2017-2016, using the polymerase chain reaction (PCR) technique and determining the sequences of nitrogen bases of PCR products amplified by the universal primers ITS1 and ITS4.   Results, obtained from the PCR amplification and analysis of the sequences of nitrogen bases of PCR-amplified products using BLAST program (Basic Local Alignment Search Tool), showed that the identified isolates are belonged to the fungi <em>Rhizoctonia solani</em>, <em>F. verticilliodes</em>, <em>F. solani </em>and <em>P. tardochrysogenum</em>. From the comparison of the sequences of nitrogen bases obtained from the identified fungal isolates with those available at the National Center for Biotechnology Information (NCBl), it was revealed that the identified fungi <em>R. solani</em> and <em>P. tardochrysogenum</em> were not previously recorded at NCBI, therefore; the identified sequences of these fungi have been deposited and recorded in GenBank database (NCBI) under the accession numbers: KX255861and KX555634. اجریت ىذه الد راسة فی مختبر فایروسات النبات التابع لکمیة الزا رعة فی جامعة کربلاء بیدؼ عزؿ و تشخیص بعض الفطریات, مف جذور بعض نباتات الطماطة النامیة فی الانفاؽ البلاستیکیة الواقعة فی احدى الم ا زرع الصح ا رویة فی محافظة کربلاء خلاؿ الموس الزا رعی 2017-2016 , باستخدا تقنیة تفاعؿ البمم ة ر المتسمسؿ (Polymerase chain reaction, PCR) و تحدید تسمسؿ القواعد النایتروجینیة لنواتج الحامض النووی المضاعفة (PCR-amplified products) بواسطة البوادیء ITS1 و ITS4 . اثبتت نتائج تحمیؿ تسمسلات الحوامض النوویة المضاعفة و باستخدا برنامج BLAST (Basic Local Alignment Search Tool) اف العزلات المشخصة ىی عائدة الى الفطریات Rhizoctonia solani و Fusarium verticilliodes و Fusarium solani و Pencillium tardochrysogenum . کما وجد مف خلاؿ مقارنة تسمسلات القواعد النایتروجینیة مع البیانات المتوف ة ر فی قاعدة المرکز الوطنی لمعمومات التقنیة الحیویة (National Center for Biotechnology Information, NCBI) اف عزلات الفطر R. solani و P. tardochrysogenum ( AQNOAH و Baghdad , عمى التوالی( المشخصة فی ىذه الد ا رسة ىی عزلات جدیدة غیر معروفة سابقا, لذا ت تسجیمیا فی المرکز الوطنی لممعمومات التقنیة الحیویة ( (NCBI تحت ارقا الادخاؿ (GenBank accession numbers) KY055374 و .KY055375 4 https://jkas.uokerbala.edu.iq/article_160239_9d8a4ca4d35c907dfef30a48a657a8b2.pdf