Journal of Kerbala for Agricultural Sciences

Experiment was conducted during the growing season of summer 2014 in a rice field at Al-Hira district in the province of Najaf to examine two factors, water type (magnetized MW and regular RW) and herbicide (Nominee and Rainbow at full and half of the recommended dose) and their effects on rice growth parameters including plant height, number of branches, fresh and dry weight. The experiment was two factorial base on Randomized Completely Block Design (RCBD) with three replicates. Results showed significant differences among all treatments in the experiment. The MW treatment significantly resulted in higher values of all parameters tested compared to the RW and weedy treatments, respectively. However, values of tested plant parameters were higher in both herbicides treatments with no significance compared to those of the MW treatment but at the same time were significantly much higher where compared to other treatments. Comparison between the herbicides showed that Nominee resulted in higher, but not significant, growth parameters compared those of the herbicide Rainbow. While, interaction among all treatments showed that the best results were from MW interacted with Nominee over other interaction treatments. Magnetized water in this study was approver to be effective treatment in supporting all growth parameters in rice crop fields.

genetic diversity of the eleven varieties of Oryza sativa L using molecular indicators collaboration with Mashkhab research center in the Najaf. Use polymerase chain reaction based the indicators Simple Sequence Repeats ,the DNA isolated from fresh leaves rice after 25 days from sowing using Genomic DNA plant kit Protocol . the amount of DNA in good range (125-589) ng/μl  purity of 1.8-1.9 . after agarose gel electrophoresis & staining with ethidium bromide. results showed that 64 alleles using 13 primers. The range of molecular size between (1015.402-115.423bp). RM3412 primer  reached the highest number of 8 alleles While the lowest number of alleles of 2 alleles recorded RM201,RM10772 primers. The Polymorphism information content (PIC), which is a reflection of the diversity of allelic and repetition allelic between varieties ranged value between (0.0830 -0.8434) . As ranged genetic dimension values ​​between) 0.0868- 0.8595 (. Record all of the primer RM3412 higher values ​​ Heterozygote reached 1. this primers (RM3412 and RM8085) to give a distinctive genetic fingerprinting of three varieties under study. The lowest genetic distance 0.3269 between varieties 2 and 3 this means there is a high degree of similarity. The genetic similarity of all varieties and compositions ranged betwee ) 0.1154 - 0.6731) depend on the genetic distance that ranging from) (0.8846 -0.3269) which refers to the large genetic diversity ranged from(88% -32%) (and this reveals a high genetic variation between varieties  making it hereditary rich sources. Results showed of the Cluster analysis to 2 major groups (Main Cluster) at the level of the similarity of  0.12.

Orseolia oryzae one dangerous pests that cause a great loss to the Oryza sativa L. crop, used markers Simple Sequence Repeats based polymerization reactions chained to the detection of resistance genes to disease. the DNA isolate of rice leaves  after 25 days from sowing .using Genomic DNA plant kit Protocol to extraction , the amount of DNA in good range (125-589) ng / μl  purity of 08.01 to 09.01. migrated samples on the gel akaros after completing the PCR program results showed three primers with sixteen cultivar ,The range of molecular size between (1156.84-197.768bp).the primer RM23956 reached to highest number of 16 alleles While the lowest number of 11 alleles recorded RM28574 primers. The Polymorphism information content (PIC) which is a reflection of the diversity of allelic and repetition allelic between varieties ranged value between ((0.8807-0.9837. As ranged gene  diversity values ​​between  0.8906- 0.9772. the primers RM23956 which reveals gane GM1 in twelve genotypes followed by the  RM28574 which reveals gane GM11 in seven genotypes either the primers RM547 which reveals gane GM4 in five genotypes, as the results showed that the  abaa1 cultivars of the contains three genes.