Journal of Kerbala for Agricultural Sciences

This study was conducted in Karbala governance‏ during 2014, for isolation and identification of some fungi from surface and digestive of Periplaneta americana L.(Orthoptera: Blattidae). The insect samples were collected from women's and obstetricians hospital and residential areas near of the hospital. The study showed isolation and identification several fungi that were Aspergillus niger, A.fumigatus , Trichoderma harzianum , Penicillium sp. , Alternaria sp. , Mucor sp. and Fusarium sp from surface and digestive of P. americana. The Aspergillus niger was the highest percentage frequency about 26.32%, compared with other fungi while the lowest percentage frequency was recorded for Mucor sp. with 1.75%. The pathogenicity test indicated to the potential effect of Trichoderma harzianum against adult of P. Americana. The T. harzianum suspensions were applied on the adult either by direct spraying or as bait. The bait method reached mortality 80% of the insect with fungal concentration 17x 107 after 10 day compared with direct spraying that was reached 40% mortality with same fungi in concentration above after same period.

Field experiment were carried out to investigate the effectiveness of some insecticides Actara (Thiamethoxam), Avaunt(Indoxacarb) and Karate(Lambda  cyhalothrin) and Bio-Agent  Trichoderma  harizaniumagainst larvae and adult stages of Radish beetle Colaphellus apicalis Mentr (Coleoptera Chrysomelidae ).The results showed that Avaunt and Actara were the best effective and gave mortality of larvae 90.42 and 88.51%, respectively .while Karate gave 80..85% mortality of larvae. The results showed almost similar effect of Avaunt and Actara against adult and gave mortality of adult 83.84and 79.4%, respectively whereas Karate gave 77.33%.The study also showed that spore suspension of Trichoderma harzianum caused a high mortality against larvae and adult stages of Colaphellus apicalis Mentr reached 61.22and 53.08%, respectively. When used at concentration 1×1012 after seven day from spraying. The results reveal that the mortality increased when exposure period was increased.